Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides

Fc region binding peptide conjugated with attenuated cationic amphiphilic lytic L17E trimer [FcB(L17E)3] was designed for immunoglobulin G (IgG) delivery into cells. Particle-like liquid droplets were generated by mixing Alexa Fluor 488 labeled IgG (Alexa488-IgG) FcB(L17E)3. Droplet contact the cellular membrane led to spontaneous influx and distribution of Alexa488-IgG throughout cells in serum containing medium. Involvement machinery accompanied actin polymerization ruffling suggested translocation. negative charges crucial droplet formation positively charged Binding FcB(L17E)3 may not be necessary. Successful intracellular 594-labeled anti-nuclear pore complex antibody anti-mCherry-nanobody tagged supernegatively green fluorescence protein allowed targets presence